5FM4
Structure of the C-terminally extended domain My4 of human myomesin (space group P21)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-25 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.530, 156.840, 48.260 |
Unit cell angles | 90.00, 94.38, 90.00 |
Refinement procedure
Resolution | 39.210 - 2.800 |
R-factor | 0.2621 |
Rwork | 0.261 |
R-free | 0.28700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fm5 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.072 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.210 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.090 | 0.310 |
Number of reflections | 15138 | |
<I/σ(I)> | 10.8 | 3.1 |
Completeness [%] | 95.3 | 89.8 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 25 % (W/V) PEG 3350 100 MM BIS-TRIS PH 5.5 |