5FLU
Structure of a Chaperone-Usher pilus reveals the molecular basis of rod uncoilin
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON II (4k x 4k) |
| Number of Images | 90 |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.8 |
| Software | SPIDER |
