5FKT
Unraveling the first step of xyloglucan degradation by the soil saprophyte Cellvibrio japonicus through the functional and structural characterization of a potent GH74 endo-xyloglucanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2015-02-14 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.120, 94.290, 105.560 |
| Unit cell angles | 90.00, 103.20, 90.00 |
Refinement procedure
| Resolution | 69.480 - 1.520 |
| R-factor | 0.16858 |
| Rwork | 0.167 |
| R-free | 0.19384 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fkr |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.891 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.480 | 1.560 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.090 | 0.720 |
| Number of reflections | 245787 | |
| <I/σ(I)> | 10.7 | 1.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M SODIUM ACETATE (PH 5.0), 0.6 M SODIUM FORMATE, 8 % W/V PGA-LM |
