5FKM
TetR(D) T103A mutant in complex with anhydrotetracycline and magnesium, I4(1)22
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RIGAKU CCD |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 68.145, 68.145, 179.517 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.160 - 1.630 |
| R-factor | 0.17187 |
| Rwork | 0.170 |
| R-free | 0.21183 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d7m |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.645 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.200 | 1.730 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.080 | 0.970 |
| Number of reflections | 26692 | |
| <I/σ(I)> | 21.9 | 2.4 |
| Completeness [%] | 99.6 | 98.1 |
| Redundancy | 10.7 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 295 | 1.2 M (NH4)2SO4, 0.1 M MES PH 6.5, 110 MM NACL, 1 MM 5A,6-ANHYDROTETRACYCLINE, 7.5 MM MGCL2 AT 295 K |
