5FKF

SAM-I riboswitch bearing the H. marismortui Kt-7 variant 3bn is UC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	173
Detector technology	PIXEL
Collection date	2013-12-08
Detector	DECTRIS PIXEL
Spacegroup name	P 43 21 2
Unit cell lengths	61.810, 61.810, 154.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.410 - 2.800
R-factor	0.2073
Rwork	0.204
R-free	0.25750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fjc
RMSD bond length	0.005
RMSD bond angle	0.985
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.470	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.090	0.520
Number of reflections	7923
<I/σ(I)>	10.5	2.9
Completeness [%]	100.0	100
Redundancy	6.2	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		40 MM NA-CACODYLATE (PH 7.0), 80 MM KCL, 50 MM BACL2, 12 MM SPERMINE-HCL, 14% (V/V) MPD