5FK8
Structure of D80A-fructofuranosidase from Xanthophyllomyces dendrorhous complexed with Neo-erlose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.568, 205.069, 146.588 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 119.250 - 1.880 |
| R-factor | 0.16836 |
| Rwork | 0.168 |
| R-free | 0.18348 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ann |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.405 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 119.250 | 1.910 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.160 | 0.590 |
| Number of reflections | 181368 | |
| <I/σ(I)> | 7.4 | 3 |
| Completeness [%] | 99.3 | 98.6 |
| Redundancy | 6.7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN WAS CRYSTALLIZED FROM 1.4M SODIUM CITRATE TRIBASIC DIHYDRATE, 0.1M HEPES PH 7.5, THEN SOAKED IN 5MM NEO-ERLOSE |
