5FK6
SAM-I riboswitch bearing the H. marismortui Kt-7 variant 3bn is CA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2014-10-13 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.170, 62.170, 153.359 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.615 - 2.500 |
| R-factor | 0.2167 |
| Rwork | 0.213 |
| R-free | 0.27550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fjc |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.616 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.170 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 1.500 |
| Number of reflections | 10933 | |
| <I/σ(I)> | 13.1 | 1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 8.8 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 40 MM NA-CACODYLATE (PH 7.0), 80 MM KCL, 50 MM BACL2, 12 MM SPERMINE-HCL, 12% (V/V) MPD |
