5FK4
SAM-I riboswitch bearing the H. marismortui Kt-7 variant 3bn is UU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2013-08-05 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.550, 57.550, 155.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.370 - 2.430 |
| R-factor | 0.1928 |
| Rwork | 0.190 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fjc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.455 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.370 | 2.490 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.060 | 0.640 |
| Number of reflections | 10550 | |
| <I/σ(I)> | 19.2 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.5 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 40 MM NA-CACODYLATE (PH 7.0), 80 MM KCL, 50 MM BACL2, 12 MM SPERMINE-HCL, 12% (V/V) MPD. |
