5FJZ
Yeast delta-COP-I mu-homology domain complexed with Dsl1 WxWxV peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-02-18 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 72.810, 72.810, 343.290 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.400 - 1.900 |
R-factor | 0.14661 |
Rwork | 0.145 |
R-free | 0.18612 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fjw |
RMSD bond length | 0.020 |
RMSD bond angle | 2.071 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.000 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.130 | 0.710 |
Number of reflections | 78953 | |
<I/σ(I)> | 11.3 | 3.4 |
Completeness [%] | 98.3 | 98.3 |
Redundancy | 9.1 | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.6 | 0.1 M HEPES PH 6.6, 2.8 M AMMONIUM SULPHATE |