5FJY
Crystal structure of mouse kinesin light chain 2 (residues 161-480)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.700, 86.280, 111.740 |
| Unit cell angles | 90.00, 98.41, 90.00 |
Refinement procedure
| Resolution | 42.880 - 4.000 |
| R-factor | 0.2343 |
| Rwork | 0.233 |
| R-free | 0.26110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3CEQ AND 3ZFW |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.880 | 4.100 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.120 | 1.220 |
| Number of reflections | 11813 | |
| <I/σ(I)> | 3.9 | 0.6 |
| Completeness [%] | 98.4 | 98.8 |
| Redundancy | 3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 8% (W/V) PGA-LM 0.3 M NA-MALONATE 0.1 M NA-CACODYLATE PH 6.5 |
