5FJX
Yeast delta-COP-I mu-homology domain complexed with Gcs1 WxxF peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-11 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.765, 84.855, 148.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.320 - 2.450 |
| R-factor | 0.20156 |
| Rwork | 0.197 |
| R-free | 0.28742 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fjw |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.616 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.100 | 0.900 |
| Number of reflections | 33682 | |
| <I/σ(I)> | 12.5 | 1.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.5 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M TRIS-HCL PH8.5, 0.2M MGCL2, 30% PEG-4000 |
