5FJQ
Structural and functional analysis of a lytic polysaccharide monooxygenase important for efficient utilization of chitin in Cellvibrio japonicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 91.500, 50.170, 114.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.750 - 1.850 |
R-factor | 0.22086 |
Rwork | 0.218 |
R-free | 0.28369 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bem |
RMSD bond length | 0.019 |
RMSD bond angle | 1.888 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.750 | 1.900 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.060 | 0.200 |
Number of reflections | 43624 | |
<I/σ(I)> | 13.1 | 5.4 |
Completeness [%] | 96.9 | 96.5 |
Redundancy | 2.25 | 2.35 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |