5FJI
Three-dimensional structures of two heavily N-glycosylated Aspergillus sp. Family GH3 beta-D-glucosidases
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2009-10-17 |
Detector | DECTRIS PIXEL |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.519, 129.673, 217.717 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 111.410 - 1.950 |
R-factor | 0.14983 |
Rwork | 0.149 |
R-free | 0.17441 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x40 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.880 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.110 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.080 | 0.440 |
Number of reflections | 176759 | |
<I/σ(I)> | 8.3 | 2.2 |
Completeness [%] | 97.0 | 91.7 |
Redundancy | 2.9 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1M MIB (PACT SCREEN BUFFER)PH 5.0, 21 % PEG 1500, 25 % ETHYLENE GLYCOL |