5FJ4
Structure of the standard kink turn HmKt-7 as stem loop bound with U1A and L7Ae proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2013-01-29 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 131.853, 161.226, 149.899 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.613 - 2.950 |
| R-factor | 0.2007 |
| Rwork | 0.199 |
| R-free | 0.23690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c4w |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.845 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.370 | 3.090 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.080 | 1.380 |
| Number of reflections | 34011 | |
| <I/σ(I)> | 23.6 | 1.1 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS HYDROCHLORIDE PH 8.5, 2.0 M MONO-AMMONIUM DIHYDROGEN PHOSPHATE |
