5FJ1
Structure of the standard kink turn HmKt-7 as stem loop in P212121 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2013-08-04 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.052, 84.360, 174.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.720 - 2.750 |
| R-factor | 0.1677 |
| Rwork | 0.167 |
| R-free | 0.18300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bw0 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.575 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.720 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.060 | 0.690 |
| Number of reflections | 32761 | |
| <I/σ(I)> | 12.4 | 1.9 |
| Completeness [%] | 99.2 | 98.5 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 60% V/V TACSIMATE PH 7.0 |
