5FJ0
Structure of the standard kink turn HmKt-7 as simple duplex in P4222 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2014-02-25 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 50.504, 50.504, 144.953 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.692 - 2.200 |
| R-factor | 0.2047 |
| Rwork | 0.203 |
| R-free | 0.23720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cs1 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.418 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.320 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.040 | 0.880 |
| Number of reflections | 10187 | |
| <I/σ(I)> | 26.6 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.2 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.04 M MAGNESIUM CHLORIDE 0.05 M SODIUM CACODYLATE PH 6.0 5% V/V 2-METHYL-2,4-PENTANEDIOL |
