5FIX
Structure of D80A-fructofuranosidase from Xanthophyllomyces dendrorhous complexed with sucrose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-27 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 74.734, 205.219, 146.071 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 119.000 - 2.010 |
R-factor | 0.16716 |
Rwork | 0.166 |
R-free | 0.18473 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ann |
RMSD bond length | 0.008 |
RMSD bond angle | 1.506 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 119.000 | 2.040 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.118 | 0.550 |
Number of reflections | 14803 | |
<I/σ(I)> | 8.2 | 2 |
Completeness [%] | 99.0 | 98.1 |
Redundancy | 6.8 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | PROTEIN WAS CRYSTALLIZED FROM 1.4M SODIUM CITRATE TRIBASIC DIHYDRATE, 0.1M HEPES PH 7.5, THEN SOAKED IN 6% W/V SUCROSE |