5FIS
Exonuclease domain-containing 1 (Exd1) in the Gd bound conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-04 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 82.860, 135.700, 51.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 1.600 |
| R-factor | 0.15818 |
| Rwork | 0.157 |
| R-free | 0.18245 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.473 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.070 | 0.820 |
| Number of reflections | 143744 | |
| <I/σ(I)> | 12 | 1.9 |
| Completeness [%] | 97.2 | 96.7 |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 10% (W/V) PEG 2K, 200 MM KCL, 50 MM NA CACODYLATE (PH=6) 2 MM GDCL3, |
