5FIF
Carboxyltransferase domain of a single-chain bacterial carboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-05 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 111.470, 149.570, 189.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.554 - 2.494 |
R-factor | 0.2031 |
Rwork | 0.202 |
R-free | 0.24180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1on3 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.602 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.554 | 2.580 |
High resolution limit [Å] | 2.494 | 2.494 |
Rmerge | 0.215 | |
Number of reflections | 110205 | |
<I/σ(I)> | 13.34 | 1.9 |
Completeness [%] | 99.6 | 97.4 |
Redundancy | 13.6 | 13.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | PEG 3350 DL-Malic acid |