5FHY
Crystal structure of FliD (HAP2) from Pseudomonas aeruginosa PAO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 6 |
| Unit cell lengths | 124.741, 124.741, 107.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.371 - 2.201 |
| R-factor | 0.251 |
| Rwork | 0.249 |
| R-free | 0.28670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | low resolution SeMet protein |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.955 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.400 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.085 | 3.702 |
| Number of reflections | 47974 | |
| <I/σ(I)> | 17.15 | 0.55 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 7.7 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Crystallized in 0.8 M NaK Tartrate, 0.1 M Hepes pH 7.5 using random matrix microseed screening |
