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5FHS

Yeast 20S proteasome beta5-K33A mutant (propeptide expressed in trans) in complex with Carfilzomib

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06SA
Synchrotron siteSLS
BeamlineX06SA
Temperature [K]100
Detector technologyPIXEL
Collection date2015-12-10
DetectorPSI PILATUS 6M
Wavelength(s)1.0
Spacegroup nameP 1 21 1
Unit cell lengths136.350, 300.770, 145.010
Unit cell angles90.00, 113.16, 90.00
Refinement procedure
Resolution15.000 - 2.700
R-factor0.18815
Rwork0.187
R-free0.21197
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1ryp
RMSD bond length0.005
RMSD bond angle0.899
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwareREFMAC
Refinement softwareREFMAC (5.7.0032)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0002.800
High resolution limit [Å]2.7002.700
Rmerge0.0720.474
Number of reflections288442
<I/σ(I)>122.9
Completeness [%]98.498.2
Redundancy3.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.829320 mM MgAC2, 13% MPD, 0.1 M MES

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