5FHK
Regulatory domain of AphB in Vibrio vulnificus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2015-06-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97960 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 124.965, 187.926, 57.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.255 - 1.905 |
R-factor | 0.2101 |
Rwork | 0.208 |
R-free | 0.25070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3szp |
RMSD bond length | 0.010 |
RMSD bond angle | 1.021 |
Data reduction software | PHENIX |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.260 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.111 | 0.639 |
Number of reflections | 90980 | |
<I/σ(I)> | 14.08 | 3.73 |
Completeness [%] | 98.1 | 85.4 |
Redundancy | 8.7 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 287 | 0.1M HEPES:NaOH (pH 7.5), 15% PEG 8000, 10% Ethylene glycol |