5FHG

Structure of unliganded Pif1 from Bacteroides sp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 41
Unit cell lengths	123.485, 123.485, 66.477
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.534 - 2.000
R-factor	0.1931
Rwork	0.190
R-free	0.25300
RMSD bond length	0.007
RMSD bond angle	0.819
Data reduction software	Aimless
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.700	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	61720
<I/σ(I)>	9.2	1.7
Completeness [%]	91.3	88.9
Redundancy	3.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	200 mM MgCl2, 0.1 M Tris, 10% PEG 8000