5FHF
Crystal structure of Bacteroides sp Pif1 in complex with ADP-AlF4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-01-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.090, 75.310, 111.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.780 - 2.150 |
R-factor | 0.213 |
Rwork | 0.211 |
R-free | 0.25080 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.977 |
Data reduction software | Aimless |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.400 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 33204 | |
<I/σ(I)> | 20.2 | 1.9 |
Completeness [%] | 98.9 | 92.5 |
Redundancy | 7.5 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 200 mM MgCl2, 0.1 M Tris, 10% PEG 8000 |