5FHE
Crystal structure of Bacteroides Pif1 bound to ssDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-31 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 87.442, 87.442, 149.822 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.792 - 2.900 |
R-factor | 0.2255 |
Rwork | 0.223 |
R-free | 0.27710 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.154 |
Data reduction software | Aimless |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.800 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 15236 | |
<I/σ(I)> | 14.6 | 2.1 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 9.4 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | 4 M sodium formate, 0.1 M HEPES, 5% glycerol |