5FHD
Structure of Bacteroides sp Pif1 complexed with tailed dsDNA resulting in ssDNA bound complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.471, 149.640, 185.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.544 - 2.000 |
R-factor | 0.1797 |
Rwork | 0.178 |
R-free | 0.21150 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.224 |
Data reduction software | Aimless |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 91717 | |
<I/σ(I)> | 27.7 | 2.7 |
Completeness [%] | 99.2 | 97 |
Redundancy | 8.6 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 18% PEG 4000, 10% isopropanol, 0.1 M HEPES, 0.2 M sodium acetate |