5FH9
Crystal structure of NFeoB from Escherichia coli BL21 in the apo state.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9085 |
Spacegroup name | P 63 |
Unit cell lengths | 166.675, 166.675, 65.581 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.115 - 3.159 |
R-factor | 0.2088 |
Rwork | 0.208 |
R-free | 0.22840 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.752 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.120 | 3.272 |
High resolution limit [Å] | 3.159 | 3.159 |
Rmerge | 0.233 | 1.384 |
Number of reflections | 18052 | |
<I/σ(I)> | 9.11 | |
Completeness [%] | 99.6 | 96.3 |
Redundancy | 7.5 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 26.9 % PEG 8000, 0.39 M MgCl2, 0.1 M Tris-HCl pH8.0 |