5FH0
The structure of rat cytosolic PEPCK variant E89A complex with GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-04 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.428, 85.710, 118.415 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.430 - 1.600 |
| R-factor | 0.2023 |
| Rwork | 0.201 |
| R-free | 0.23160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dt2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.480 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
| Rmerge | 0.066 | 0.047 | 0.344 |
| Rmeas | 0.068 | 0.049 | 0.361 |
| Rpim | 0.018 | 0.013 | 0.107 |
| Total number of observations | 1129880 | ||
| Number of reflections | 81130 | ||
| <I/σ(I)> | 16.8 | ||
| Completeness [%] | 99.2 | 98.6 | 92.9 |
| Redundancy | 13.9 | 14.1 | 10.8 |
| CC(1/2) | 0.999 | 0.970 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 25% PEG 3350, 0.1M HEPES PH 7.4, 8MM MNCL2, 10mM GTP |
