5FGU
Structure of Sda1 nuclease apoprotein as an EGFP fixed-arm fusion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 93.15 |
Detector technology | CCD |
Collection date | 2015-02-19 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 81.407, 139.373, 120.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.276 - 1.896 |
R-factor | 0.178 |
Rwork | 0.177 |
R-free | 0.20120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | EGFP component of 4JRB |
RMSD bond length | 0.011 |
RMSD bond angle | 1.217 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.896 | 1.896 |
Rmerge | 0.610 | |
Number of reflections | 54586 | |
<I/σ(I)> | 25.6 | 3.54 |
Completeness [%] | 100.0 | 99.6 |
Redundancy | 7.3 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | Crystals were grown by mixing 0.25uL of protein (13.3mg/mL) with 0.25uL mother liquor (45mM Na cacodylate pH 6, 13.5mM magnesium sulfate, 1.53M ammonium sulfate), using sitting drop vapor diffusion |