5FGS
Crystal structure of C-terminal domain of shaft pilin spaA from Lactobacillus rhamnosus GG - P21212 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | Xenocs GeniX 3D Cu HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-03-21 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 57.070, 74.343, 116.777 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.170 - 2.000 |
R-factor | 0.21816 |
Rwork | 0.217 |
R-free | 0.24970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fgr |
RMSD bond length | 0.008 |
RMSD bond angle | 1.165 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.170 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.063 | 0.649 |
Number of reflections | 33984 | |
<I/σ(I)> | 24.2 | 2.9 |
Completeness [%] | 99.3 | 97 |
Redundancy | 9.2 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1M MES, 0.02M zinc sulphate, 25% PEG 550MME |