5FGP
Crystal structure of D. melanogaster Pur-alpha repeat I-II in complex with DNA.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8726 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 81.940, 40.190, 48.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.934 - 2.000 |
R-factor | 0.1656 |
Rwork | 0.163 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k44 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.250 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1760) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.125 | 0.793 |
Number of reflections | 11349 | |
<I/σ(I)> | 18.85 | 2.61 |
Completeness [%] | 99.4 | 94.3 |
Redundancy | 13.1 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 50 mM MES pH 5.2, 500 mM (NH4)2SO4, 1 mM TCEP and 16 % PEG400 |