5FGO
Crystal structure of D. melanogaster Pur-alpha repeat III.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.460, 55.530, 67.840 |
| Unit cell angles | 90.00, 95.65, 90.00 |
Refinement procedure
| Resolution | 47.725 - 2.600 |
| R-factor | 0.206 |
| Rwork | 0.202 |
| R-free | 0.28850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n8b |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.357 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.650 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.110 | 0.680 |
| Number of reflections | 13999 | |
| <I/σ(I)> | 19.4 | 1.97 |
| Completeness [%] | 96.5 | 98.5 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 50 mM MES pH 6.5, 200 mM NaCl, 16% PEG 3350 and 6 % MPD |
