5FGC
Three dimensional structure of broadly neutralizing human anti - Hepatitis C virus (HCV) glycoprotein E2 Fab fragment HC33.8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2014-06-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00002 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 66.050, 49.570, 66.390 |
Unit cell angles | 90.00, 98.50, 90.00 |
Refinement procedure
Resolution | 49.570 - 1.900 |
R-factor | 0.1962 |
Rwork | 0.194 |
R-free | 0.23630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB |
RMSD bond length | 0.010 |
RMSD bond angle | 1.160 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 33283 | |
<I/σ(I)> | 16.39 | 1.21 |
Completeness [%] | 99.7 | 97.8 |
Redundancy | 6.7 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 32% PEG 4000 100 mM Tris-HCl pH 8.5 800 mM lithium chloride Crystals obtained using heterologous microseeding. |