5FFZ
S. aureus MepR bound to ethidium bromide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-04-27 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.54 |
Spacegroup name | H 3 2 |
Unit cell lengths | 119.061, 119.061, 56.279 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.015 - 2.585 |
R-factor | 0.2479 |
Rwork | 0.245 |
R-free | 0.26990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eco |
RMSD bond length | 0.004 |
RMSD bond angle | 0.874 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.585 | 2.585 |
Rmerge | 0.052 | 0.461 |
Number of reflections | 4865 | |
<I/σ(I)> | 19.32 | 3.08 |
Completeness [%] | 99.4 | 93.93 |
Redundancy | 8.1 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 4M potassium formate, 0.1 M BisTris propane, pH 9, 2% PEG MME 2000 |