5FFL
Crystal structure of mouse CD300lf at 1.6 Angstroms resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.633, 53.347, 70.516 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.191 - 1.602 |
| R-factor | 0.1631 |
| Rwork | 0.160 |
| R-free | 0.19610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xed |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.102 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.6.0) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 1.670 |
| High resolution limit [Å] | 1.600 | 3.200 | 1.600 |
| Rmerge | 0.055 | 0.040 | 0.497 |
| Total number of observations | 74844 | ||
| Number of reflections | 13686 | ||
| <I/σ(I)> | 24.8 | ||
| Completeness [%] | 95.6 | 91 | 88.3 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.25 | 293 | 35% (v/v) 2-methyl-2,4-pentanediol, 50 mM sodium chloride, 120 mM Tris-HCL at pH 7.25 |
