5FFB
CopM in the apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 53.867, 86.115, 33.442 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.981 - 1.702 |
R-factor | 0.1833 |
Rwork | 0.181 |
R-free | 0.21850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bt5 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.006 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.484 | |
Number of reflections | 17514 | |
<I/σ(I)> | 15 | 2.7 |
Completeness [%] | 98.7 | 93.9 |
Redundancy | 6.5 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1M HEPES 7.0, 0.1M Lithium sulphate, 25% PEG 3350 |