Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97924 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.520, 117.250, 67.420 |
| Unit cell angles | 90.00, 94.34, 90.00 |
Refinement procedure
| Resolution | 44.180 - 3.070 |
| R-factor | 0.229 |
| Rwork | 0.228 |
| R-free | 0.24680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.800 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.180 |
| High resolution limit [Å] | 3.070 | 3.070 |
| Rmerge | 0.847 | |
| Number of reflections | 27515 | |
| <I/σ(I)> | 15.3 | |
| Completeness [%] | 99.4 | 99.2 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10% PEG 5000 MME, 5% TACSIMATE, 0.1 M HEPES, 15 MM SPERMIDINE, 25% GLYCEROL, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K, TEMPERATURE 293.0K |
