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5FEL

Hen egg lysozyme at room temperature solved from dataset acquired by oscillation method

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06SA
Synchrotron siteSLS
BeamlineX06SA
Temperature [K]293
Detector technologyPIXEL
Collection date2015-07-13
DetectorDECTRIS PILATUS 6M
Wavelength(s)1.0
Spacegroup nameP 43 21 2
Unit cell lengths80.020, 80.020, 37.690
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution40.010 - 1.800
R-factor0.15218
Rwork0.150
R-free0.19893
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4xjb
RMSD bond length0.023
RMSD bond angle2.036
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwareMOLREP
Refinement softwareREFMAC (5.8.0124)
Data quality characteristics
 Overall
Low resolution limit [Å]50.000
High resolution limit [Å]1.200
Rmerge0.063
Number of reflections72771
<I/σ(I)>11.19
Completeness [%]99.9
Redundancy6.56
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP4.5293Na acetate, MPEG 5K, NaCl, Na acetate, ethylene glycerol

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