5FEJ
CopM in the Cu(I)-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.824, 86.082, 74.971 |
| Unit cell angles | 90.00, 106.26, 90.00 |
Refinement procedure
| Resolution | 36.939 - 2.500 |
| R-factor | 0.2133 |
| Rwork | 0.211 |
| R-free | 0.25860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bt5 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.703 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.521 | |
| Number of reflections | 22448 | |
| <I/σ(I)> | 10.1 | 2.2 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1M MES 6.0, 20% PEG 2000 MME, 1mM CuSO4, 10mM Vitamin C |
