5FEI
Crystal structure of the bacteriophage phi29 tail knob protein gp9 truncation variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 160 |
Detector technology | CCD |
Collection date | 2013-09-18 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 3 |
Unit cell lengths | 184.671, 184.671, 184.671 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.355 - 2.604 |
R-factor | 0.1682 |
Rwork | 0.166 |
R-free | 0.20070 |
Structure solution method | SIRAS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.179 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 130.582 |
High resolution limit [Å] | 2.604 |
Number of reflections | 64249 |
<I/σ(I)> | 16.49 |
Completeness [%] | 99.9 |
Redundancy | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 293.15 | 1.2M lithium sulfate, 10% v/v PEG 400, sodium acetate buffer, pH 4.4 |