5FDN
Crystal structure of phosphoenolpyruvate carboxylase from Arabidopsis thaliana in complex with aspartate and citrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03323 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 264.260, 268.130, 77.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.870 - 2.200 |
R-factor | 0.1734 |
Rwork | 0.173 |
R-free | 0.19250 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.947 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.870 | 2.500 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.239 | |
Number of reflections | 140308 | |
<I/σ(I)> | 19.41 | 8.48 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.4 | 7.52 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 6% PEG 6000, 100 mM sodium citrate pH 4.6 |