5FDK
Crystal structure of RecU(D88N) in complex with palindromic DNA duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-12-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 144.447, 144.447, 310.343 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.694 - 3.208 |
| R-factor | 0.2597 |
| Rwork | 0.256 |
| R-free | 0.32860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.657 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER (2.5.3) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 116.024 | 48.694 | 3.380 |
| High resolution limit [Å] | 3.208 | 10.140 | 3.210 |
| Rmerge | 0.091 | 0.033 | 1.415 |
| Rmeas | 0.100 | ||
| Rpim | 0.030 | 0.012 | 0.440 |
| Total number of observations | 229929 | 6557 | 33396 |
| Number of reflections | 20730 | ||
| <I/σ(I)> | 16.7 | 54.8 | 1.8 |
| Completeness [%] | 99.8 | 96 | 99.4 |
| Redundancy | 11.1 | 9.5 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 290 | 0.1 M BICINE pH 9, 10% PEG 6000 |
