5FD8
Crystal Structure of MccF-like Protein (BA_5613) in complex with ASA (alanyl sulfamoyl adenylates)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.777, 83.683, 130.845 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.309 - 2.050 |
R-factor | 0.1708 |
Rwork | 0.169 |
R-free | 0.20040 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.731 |
Data scaling software | HKL-3000 |
Phasing software | SHELX |
Refinement software | PHENIX (dev_1888) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.310 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.120 | 0.790 |
Number of reflections | 37167 | |
<I/σ(I)> | 9 | 2.2 |
Completeness [%] | 90.0 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 293 | 30.0 % w/v PEG 3000 50 mM Bicine |