5FD4
Crystal Structure of ComR from Streptococcus suis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-06-11 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.97851 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 82.271, 82.271, 209.753 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.500 - 2.900 |
R-factor | 0.21388 |
Rwork | 0.212 |
R-free | 0.24208 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.096 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.500 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.081 | 1.116 |
Number of reflections | 18768 | |
<I/σ(I)> | 12.3 | 1.2 |
Completeness [%] | 99.3 | 98.7 |
Redundancy | 5.9 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M Sodium Citrate, 15% PEG 3350 dehydration O/N with 35% PEG 8000 |