5FCY

Structure of Anabaena (Nostoc) sp. PCC 7120 Red Carotenoid Protein binding a mixture of carotenoids

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00003
Spacegroup name	P 32
Unit cell lengths	58.373, 58.373, 42.251
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.419 - 2.508
R-factor	0.2378
Rwork	0.232
R-free	0.29210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xb4
RMSD bond length	0.003
RMSD bond angle	0.719
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.419	2.640
High resolution limit [Å]	2.508	2.510
Rmerge	0.031	0.156
Number of reflections	5403
<I/σ(I)>	34.7	5.1
Completeness [%]	98.0	83.7
Redundancy	5.2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	100 mM HEPES pH 7.5, 10% 2-propanol, 25% PEG 4000