5FCX
Structure of Anabaena (Nostoc) sp. PCC 7120 Red Carotenoid Protein binding canthaxanthin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976484 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 159.505, 159.505, 159.505 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.686 - 3.206 |
| R-factor | 0.2132 |
| Rwork | 0.208 |
| R-free | 0.26060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xb4 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.712 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.690 | 3.280 |
| High resolution limit [Å] | 3.206 | 3.206 |
| Rmerge | 0.481 | 1.916 |
| Number of reflections | 11920 | |
| <I/σ(I)> | 8.9 | 2 |
| Completeness [%] | 99.7 | 98.4 |
| Redundancy | 20.7 | 20.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 400 mM ammonium citrate, 20% PEG 3350 |
