5FCR
MOUSE COMPLEMENT FACTOR D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-08-16 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 1 |
Unit cell lengths | 51.271, 52.866, 84.278 |
Unit cell angles | 90.46, 98.04, 90.14 |
Refinement procedure
Resolution | 83.450 - 1.250 |
R-factor | 0.15209 |
Rwork | 0.150 |
R-free | 0.19599 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fck |
RMSD bond length | 0.032 |
RMSD bond angle | 2.700 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.350 | 1.280 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.040 | 0.720 |
Number of reflections | 215515 | |
<I/σ(I)> | 13.9 | |
Completeness [%] | 88.3 | 75.1 |
Redundancy | 1.8 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Mouse FD was crystallized by the sitting drop vapour diffusion method. A 1 uL FD solution (14.4 mg/mL FD, 50 mM Tris pH 8.0, 100 mM NaCl) was mixed with 1 uL reservoir solution (0.1 M HEPES pH 7.0, 30% v/v Jeffamine ED-2001 pH 7.0) and equilibrated against 200 uL reservoir solution. |