5FCH

Crystal Structure of Xaa-Pro dipeptidase from Xanthomonas campestris, phosphate and Zn bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE BL-21
Synchrotron site	RRCAT INDUS-2
Beamline	BL-21
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97947
Spacegroup name	P 21 21 21
Unit cell lengths	82.032, 104.080, 112.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 1.950
R-factor	0.17362
Rwork	0.172
R-free	0.21087
RMSD bond length	0.026
RMSD bond angle	2.234
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.220	1.990
High resolution limit [Å]	1.950	1.950
Rmerge	0.107	0.798
Number of reflections	70802
<I/σ(I)>	13.6	2.4
Completeness [%]	100.0	99.9
Redundancy	6.1	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		294	40mM KH2PO4, 20% Glycerol, 14% PEG8000, 2mM ZnCl2