5FBP
CRYSTAL STRUCTURE OF THE NEUTRAL FORM OF FRUCTOSE-1,6-BISPHOSPHATASE COMPLEXED WITH THE PRODUCT FRUCTOSE 6-PHOSPHATE AT 2.1-ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 131.100, 131.100, 69.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 * - 2.100 |
| R-factor | 0.177 * |
| Rwork | 0.177 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.900 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.100 * |
| Total number of observations | 200580 * |
| Number of reflections | 38643 * |
| Completeness [%] | 97.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | buttom | enzyme | 13 (mg/ml) | |
| 2 | 1 | reservior | Tris-HCl | 20 (mM) | |
| 3 | 1 | reservoir | maleic acid | 2 (mM) | |
| 4 | 1 | reservoir | EDTA | 0.1 (mM) | |
| 5 | 1 | reservoir | 5 (mM) |
