5FBM

Crystal Structure of Histone Like Protein (HLP) from Streptococcus mutans Refined to 1.9 A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	32.458, 76.333, 36.933
Unit cell angles	90.00, 107.21, 90.00

Refinement procedure

Resolution	38.166 - 1.900
R-factor	0.1785
Rwork	0.177
R-free	0.21670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rhi
RMSD bond length	0.010
RMSD bond angle	0.962
Data reduction software	XDS
Data scaling software	Aimless (0.2.14)
Phasing software	PHASER (2.5.5)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.170	38.170	1.940
High resolution limit [Å]	1.900	9.110	1.900
Rmerge	0.051	0.026	0.714
Rpim	0.033	0.017	0.454
Total number of observations	45407	391	2971
Number of reflections	13437
<I/σ(I)>	13.8	40.1	1.9
Completeness [%]	98.8	87	99.4
Redundancy	3.4	3.3	3.4
CC(1/2)	0.999	0.998	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	293	30% PEG 400 and 0.1M CAPS